Features of LAMBDA PERISTALTIC PUMPS
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LAMBDA pump mimics the features of an Ideal Peristaltic pump
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Rollers of large diameter which gives soft compression over tubing
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No tubing leakage
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Reduced pulsation and stable flow rate for many days
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With RS-485 interface
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Flow rate: 200 nL/min – 50 L/hr
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Weighs less than 1 Kg
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Fully automated and remote controllable
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Inbuilt integrator - To access the amount of fluid transferred
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A pump that fits in your hand
PERISTALTIC PUMPS PRECIFLOW-MULTIFLOW-HiFLOW-MAXIFLOW-MEGAFLOW OVERVIEW
LAMBDA PRECIFLOW, MULTIFLOW, HIFLOW and MAXIFLOW peristaltic pumps have been developed for continuous cultures as the result of twenty years of laboratory experience and involved the systematic elimination of the imperfections found in other pumps on the market. The special mechanics of LAMBDA peristaltic pumps has been developed because no pump could be used for continuous fermentation lasting up to eight weeks.
The most frequent problems of traditional peristaltic pumps are:
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Aspiration of the tubing by the mechanics
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Rupture of the tubing and leaking of the solution
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Flow rate decrease over time
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When a continuous process is interrupted a lot of time and money are lost
During the development of the new LAMBDA peristaltic pump mechanics all aspects of existing peristaltic pumps have been analyzed and finally an efficient solution has been found.
What makes the LAMBDA pumps so efficient?
Rollers of small diameter apply a high strain onto the tubing and push the tubing in the direction of the sense of rotation. Therefore, the tubing has to be fitted with stoppers. We use rollers of very large diameter – this leads to the elimination of such strains and prevents tubing motion. A larger portion of tubing is compressed with better pressure distribution. The elasticity of the tubing is protected.
Instead of rollers we use special plastic ball bearings with glass beads. The gliding of such rollers requires only a minimal force and the corrosion resistance in the case of spill is excellent.
A special off-centre lever using a spring of non-corrosive material applies the compression force on the tubing softly and gradually.
The final pressure of the liquid is reduced by the spring to 0.1–0.2 MPa (according to the tubing used). The pressure does not increase even when the line is blocked.
The pump head is large and has two centers of asymmetry, which reduces pulsation several times. The head is made of hard, chemically stabilized material
The stepping motor or BLDC motor, driven by quartz-controlled electronics, assures a high precision of the flow rate. The ratio of speed control is 1:1000. With several possibilities of remote control.
The unique flow integrator LAMBDA INTEGRATOR makes the LAMBDA peristaltic pumps excellent for the use in automatically controlled systems e.g. fermentation, bio cultures, chemical synthesis, fraction collection and many more.
The laboratory space is very expensive, therefore we have made our peristaltic pumps as compact as possible (all have just 1 l of volume). They are several times smaller than other peristaltic pumps on the market and therefore so handy in use.
Benefits of great importance for your laboratory
Since the lateral forces on the tubing have been eliminated, no stoppers are required. Even without any fixation the tubing will not move at all in and out of the head.
Since the tubing compression is kept in the range of elasticity of the tubing the tubing life increases considerably and flow rates remain constant.
Low cost tubing can be used with good results. The tubing economy is very important, e.g. the LAMBDA PRECIFLOW peristaltic pump will be paid back after consumption of about 80 m of tubing.
All LAMBDA peristaltic pumps have the same following characteristics:
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Digital speed control in the range 0:1000
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Analog remote control ON/OFF and full range speed control (0–10 V)
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RS 485 or RS 232 interface (optional)
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Very small dimensions: 10.5 (W) × 9.5 (H) × 10.5 (D) cm
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Very quiet operation
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Plug in switching power supply for mains 90–240 V/AC, 50–60 Hz, output 12 V/DC
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Long life even with low cost tubing without stoppers
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Safety conform to CE and IEC 1010/1
Electronic integrator LAMBDA INTEGRATOR
The use of the electronic flow integrator LAMBDA INTEGRATOR together with the LAMBDA peristaltic pumps allows a simple but precise integration of the amount of liquid, which has been transported by the pump..
The electric impulses, which move the motor, are registered and transformed into a direct current. The voltage can be measured or recorded by common recorders or voltmeters. The RS 485 or RS 232 interface allows the connection to a PC.
In processes where the pump is controlled e.g. by a pH-stat during a fermentation (to keep the pH of the medium constant), it is frequently important to know when and how much acid or base were added. This data yields important information about the process, its kinetics and time of completion..
The Integrator can also be used for measuring enzyme activity (e.g. esterases, amidases, acylases, lipases, proteases, lactamases and other enzymes).
The pump-flow integrator can be conveniently placed under the peristaltic pump. The Integrator connected to LAMBDA peristaltic pumps allows new and unusual applications of peristaltic pumps (gradient making, counter flow elution, liquid chromatography, electronic burette, etc.)
Features |
PRECIFLOW |
MULTIFLOW |
HIFLOW |
MAXIFLOW |
MEGAFLOW |
Flow Rate |
200 nL/min to 600 mL/hour |
200 nL/min to 600 mL/hour |
50 µL/min to 3 L/ hour |
200 µL/min to 10 L/ hour |
200 µL/min to 50 L/ hour |
Weight |
<1 Kg |
<1 Kg |
1.2 Kg |
1.2 Kg |
2 kg |
Programming Mode |
No Programming |
up to 99 steps |
up to 99 steps |
up to 99 steps |
up to 99 steps |
Time Resolution |
|
up to 99 steps of speed and time |
up to 99 steps of speed and time |
up to 99 steps of speed and time |
up to 99 steps of speed and time |
Motor Type |
Stepping Motor |
Stepping Motor |
BLDC Motor with neodymium magnets |
BLDC Motor with neodymium magnets |
BLDC Motor with neodymium magnets |
Tubing |
Silicone tubing or other materials having similar elasticity; inner tubing diameter from 0.5 to 4 mm and tubing wall thickness of approx. 1 mm |
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Memory |
storage of all settings |
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Maximum Pressure |
approx. 0.1 MPa in clockwise rotation; approx. 0.15 MPa in counter-clockwise rotation |
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Dimension |
10.5 (W) x 9.5 (H) x 10.5 (D) cm |
10.5 (W) x 9.5 (H) x 10.5 (D) cm |
10.5 (W) x 9.5 (H) x 10.5 (D) cm |
10.5 (W) x 9.5 (H) x 10.5 (D) cm |
10.5 (W) x 9.5 (H) x 10.5 (D) cm |
Operation Temperature |
0-40 °C |
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Operation Humidity |
0-90% RH, not condensing |
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Power Supply |
95–240 V/60–50 Hz AC plug-in power supply with DC 12V/6W output; possible field operation on 12 V accumulator |
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Remote Control |
0-10 V; (option 0-20 or 4-20 mA) |
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Warranty |
5 years |
5 years |
2 years |
2 years |
2 years |
Quotation |
Applications and short references of publications using LAMBDA peristaltic pump.
LAMBDA peristaltic pump - a new solution for liquid handling
LAMBDA Peristaltic pumps are ideal to handle liquids from micro fluids to large volumes.
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Fermentation technology and process optimization
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Cell culturing
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Transfer of medium to the fermentor or bioreactor (at high speed)
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Addition of substrate, trace elements and micro elements at low speed (200 nL/min)
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Batch, fed-batch culture, continuous culture, chemostat, turbidostat and other fermentation process.
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Perfect for feeding and harvesting of medium, substrate or cells.
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Easy to maintain the pH ( very precise addition )
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Antifoam control (addition of antifoam agent)
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Sampling at particular time interval
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Feed flow rate profile
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Sample addition/injection
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LAMBDA integrator allows to measure the pH measurement based growth kinetics.
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Integrator allows us to measure the fluid transfer with respect to time.
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Preparation of agar plates
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For Microbial Limit Test
Short reference of publications
{slider Bioprocessing}
Feed pump: Concentrated nutrient solutions was fed by LAMBDA Peristaltic Pump for the production of Erythromycin from the strain Saccharopolyspora erythraea in fed-batch
Potvin, Jean; Peringer, Paul: Ammonium regulation in Saccharopolyspora erythraea Part II Regulatory effects under different nutritional conditions. Biotechnology Letters. 1994, 16(1): 69-74
Zeneca Bio Products, Canada
Keywords: erythromycin, Saccharopolyspora erythraea, antibiotic, fermentation, growth rate, feeding, fed-batch
Feed pump: In a continuous mode of culture, the medium was supplied by PRECIFLOW peristaltic pump operated on controlled set-points for designated dilution rates (D).
Ester Martinez-Porqueras, Simon Rittmann, Christoph Herwig, Analysis of H2 to CO2 yield and physiological key parameters of Enterobacter aerogenes and Caldicellulosiruptor saccharolyticus, International Journal of Hydrogen Energy 38 (2013) 10245 to 10251.
Vienna University of Technology, Institute of Chemical Engineering, Austria.
Keywords: Biohydrogen, Biofuel, batch, repetitive batch, Chemostat culture, anaerobic, Glucose, Xylose, Hydrogen productivity, Hydrogen to carbon dioxide yield, Hydrogen to substrate yield, volumetric hydrogen evolution rate (HER), specific hydrogen evolution rate (qH2)
Feed Pump: Medium was continuously supplied to the bioreactor by PRECIFLOW Peristaltic pump with controlled flow to obtain the desired dilution rate (D)
Ester Martinez-Porqueras, Patrick Wechselberger, Christoph Herwig, Effect of medium composition on biohydrogen production by the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus, International Journal of Hydrogen Energy, Volume 38, Issue 27, 10 September 2013, Pages 11756–11764
Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering, Austria
Keywords: Caldicellulosiruptor saccharolyticus, Defined medium, Complex medium, Xylose, Double nutrient limitation, Hydrogen productivity, batch mode
PRECIFLOW Peristaltic Pump was used to supply feed medium into the bioreactor and the feed flow rate was kept constant by controlling the pump speed to get a medium dilution rate ( D ) of 0.05 per hour (h-1)
A.H. Seifert, S. Rittmann, S. Bernacchi, C. Herwig, Method for assessing the impact of emission gasses on physiology and productivity in biological methanogenesis, Bioresource Technology, Volume 136, May 2013, Pages 747–751
Vienna University of Technology, Institute of Chemical Engineering, Research Area Biochemical Engineering, Austria.
Keywords: Biomethane production, Industrial and biogenic waste gas, Biological methanogenesis, Bioreactor, Thermophilic culture, Methanothermobacter marburgensis, fermentation, anaerobic
PRECIFLOW Peristaltic Pump was used as a feed pump for the production of 2-phenylethanol (PEA) by in situ product removal (ISPR) method
D. Stark, H. Kornmann, T. Munch, B. Sonnleitner, I. W. Marison, U. von Stockar, Novel Type of In Situ Extraction: Use of Solvent Containing Microcapsules for the Bioconversion of 2-Phenylethanol from L-Phenylalanine by Saccharomyces cerevisiae, Biotechnol Bioeng. 2003 Aug 20; 83(4):376-85.
Laboratory of Chemical and Biochemical Engineering, Swiss Federal Institute of Technology (EPFL), Switzerland.
Keywords: in-situ product recovery, microencapsulation of solvent, 2-phenylethanol, extractive fermentation, mass transfer, fermenter, fed-batch culture
pH kept at 4 by the controlled addition of acid or base using PRECIFLOW Peristaltic pump to estimate the biomass production by pH control analysis
A Vicente, J I Castrillo, J A Teixeira, U Ugalde, On-line estimation of biomass through pH control analysis in aerobic yeast fermentation systems, Biotechnol Bioeng. 1998 May 20; 58(4):445-50.
Departamento de Engenharia Biológica, Universidade do Minho, Portugal and Unidad de Bioqu?´mica, Departamento de Qu?´mica Aplicada, Facultad de Ciencias Quimicas, Universidad del País Vasco, Spain
Keywords: on-line control; pH control; growth monitoring; proton titration; yeast; aerobic fermentation; chemostat
Programmable pump MULTIFLOW used as an automatic control pump to control oxygen consumption and temperature by feeding
Oxygen and Temperature Control during the Cultivation of Microorganisms using Substrate Feeding, Engineering in Life Sciences, 2007, Volume 7, Issue 3, pages 247–252.
“Biotehniskais centrs”, JSC, Latvia; Institute of Chemical Technology Prague, Czech Republic; Latvian State Institute of Wood Chemistry, Latvia.
Keywords: Bacteria; Bioreactors; Fermentation; Process control; substrate feeding; temperature control; oxygen consumption.
pH was maintained at 3.5 (±0.01) by LAMBDA PRECIFLOW Peristaltic Pump in whey chemostat culture
Juan I. Castrillo, Unai O. Ugalde, Patterns of energy metabolism and growth kinetics of Kluyveromyces marxianus in whey chemostat culture, Applied Microbiology and Biotechnology, November 1993, Volume 40, Issue 2-3, pp 386-393.
Unit of Biochemistry, Department of Applied Chemistry, Faculty of Chemistry, University of the Basque Country, Spain
Keywords: Biomass, whey, chemostat culture, Kluyveromyces marxianus, aerobic
PRECIFLOW pump continuously supplied medium with controlled set-points for designated medium dilution rates of Methanothermobacter marburgensis grown in continuous cultures
S. Rittmann, A. Seifert, C. Herwig, Quantitative analysis of media dilution rate effects on Methanothermobacter marburgensis grown in continuous culture on H22 and CO2. Biomass and Bioenergy, Volume 36, January 2012, Pages 293–301
Technische Universität Wien, Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Austria.
Keywords: Methanogenesis, Methanothermobacter marburgensis, Dynamic experiment, Continuous culture, Liquid dilution rate
Digital PRECIFLOW peristaltic pumps were used as feed pump, bleed pump and cell-free harvest pump to maximize the productivity of extreme halophilic archaeon in a bioreactor equipped with an external cell retention system
Bettina Lorantfy, Paul Ruschitzka, Christoph Herwig, Investigation of physiological limits and conditions for robustbioprocessing of an extreme halophilic archaeon using externalcell retention system, Biochemical Engineering Journal 90 (2014) 140–148.
Vienna University of Technology, Institute of Chemical Engineering, Austria.
Keywords: Halophilic Archaea, Microfiltration for cell retention, Integrated processing, Bioprocess design, Bioprocess monitoring, Ultrafiltration
{slider Metabolic Engineering}
PRECIFLOW Peristaltic Pump was used to maintain constant pH for the accurate quantitative determination of net proton production or consumption in chemostat cultures of Candida utilis
CASTRILLO, I. DE MIGUEL AND U. 0. UGALDE, Proton Production and Consumption Pathways in Yeast Metabolism. A Chemostat Culture Analysis, YEAST VOL. 11: 1353-1365 (1995)
Unit of Biochemistry, Department of Applied Chemistry, University of the Basque Country, Spain
Keywords: yeast; nitrogen pathway; chemostat culture; proton production; pH; metabolic model; control
Feed flow rate was kept constant by controlling the speed of the PRECIFLOW peristaltic pump in continuous culture of Methanothermobacter marburgensis
A.H. Seifert, S. Rittmann, C. Herwig, Analysis of process related factors to increase volumetric productivity and quality of biomethane with Methanothermobacter marburgensis, Applied Energy 132 (2014) 155–162
Vienna University of Technology, Institute of Chemical Engineering, Austria.
Keywords: Archaea, Biological methanogenesis, Continuous culture, Gas/liquid mass transfer, CO2, H2, CH4, quantification
{slider Purification}
Two computer-controlled programmable HIFLOW Peristaltic Pumps were used for gradient generation for the purification of isolated human islets
Andrew Friberg, Magnus Stahle, Olle Korsgren, Daniel Brandhorst, Human islet purification utilizing a semi-closed automated pump system, Cell Transplant, 2008;17(12):1305-13.
University Hospital, Department of Oncology, Radiology & Clinical Immunology, Uppsala, Sweden.
Keywords: Human islet isolation; Islet purification; Density gradient purification; Closed system.
LAMBDA MULTIFLOW Peristaltic Pump used to study the effect of ?ow rate on the analytes retention in range of 1.0 - 6.0 mL/min and eluent ?ow rate from 0.5 to 2.0 mL/min
Alireza Asghari, Bahram Mohammadi, Nano-alumina coated with sodium dodecyl sulfate and modified with 4-(2-Pyridylazo) resorcinol for extraction of heavy metals in different matrixes, Journal of Industrial and Engineering Chemistry, Volume 20, Issue 3, 25 May 2014, Pages 824-829
Department of Chemistry, Semnan University, Iran.
Keywords: Nano-alumina; 4-(2-Pyridylazo) resorcinol; Sodium dodecyl sulfate; Inductively coupled plasma-optical emission spectrometry; Solid phase extraction
{slider Cell Culture}
Feed and harvest pump: Medium was continuously fed into the reactor and permeate was continuously withdrawn from inside the spin-filter using two LAMBDA PRECIFLOW peristaltic pumps in an animal cell perfusion culture experiment
F. Vallez-Chetreanu, L.G. Fraisse Ferreira, R. Rabe, U. von Stockar, I.W. Marison, An on-line method for the reduction of fouling of spin-filters for animal cell perfusion cultures, Journal of Biotechnology 130 (2007) 265–273
Ecole Polytechnique F´ed´erale de Lausanne (EPFL), Laboratory of Chemical and Biochemical Engineering, Switzerland and Dublin City University, School of Biotechnology, Ireland
Keywords: Animal cell; Perfusion culture; Spin-filter; Fouling; Vibration; Piezo-electric
{slider Veterinary application}
LAMBDA Peristaltic pump was used to induce the milk flow into the Teflon chamber with stainless steel chips (at flow rate of 340 mL/h and 980 mL/h) to study Staphylococcus epidermidis adherence
Zoran Jaglic, Dana Cervinkova, Elleni Michu, Martina Holasova, Petr Roubal, Hana Vlkova, Vladimir Babak, Jarmila Schlegelova, Effect of milk temperature and flow on the adherence of Staphylococcus epidermidis to stainless steel in amounts capable of biofilm formation, Dairy Sci. & Technol. (2011) 91:361–372
Veterinary Research Institute and MILCOM a.s., Dairy Research Institute, Czech Republic
Keywords: Food safety, Staphylococcus epidermidis, Hygiene, Dairy, biofilm, adherence
{slider Bioremediation and Biodegradation}
LAMBDA MULTIFLOW pumped the simulated waste water containing dissolved dye through the reactor with the immobilized TiO2 to study the degradation of textile dyes
Jan Šíma, Pavel Hasal, Photocatalytic Degradation of Textile Dyes in aTiO2/UV System, Chemical Engineering Transactions, 2013, 32, 79-84.
Department of Chemical Engineering, Institute of Chemical Technology, Prague, Czech Republic
Keywords: TiO2 immobilization, decolorization, wastewater remediation, polyacrylamide (PA), UV, polyvinyl alcohol (PVA), photodegradation, photoreactor
The in-fluent medium was pumped using PRECIFLOW pumps to the columns containing Dehalococcoides for PCE bioremediation
Elsa Lacroix, Alessandro Brovelli, Julien Maillard, Emmanuelle Rohrbach-Brandt, D.A. Barry, Christof Holliger, Use of silicate minerals for long-term pH control during reductive dechlorination of high tetrachloroethene concentrations in continuous flow-through columns, Science of The Total Environment, Volumes 482–483, 1 June 2014, Pages 23–35
Laboratory for Environmental Biotechnology, Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland
Keywords: Organohalide respiration, Microbial reductive dechlorination, PCE, Acidification, pH buffer, Silicate minerals, bioremediation
Flexible polyethylene tube for PRECIFLOW feed pump used in the optimal growth of algae
Legendre, A and Desmazieres, N. 2011. Device for Cultivating Algae and/or Microorganisms for Treating an Effluent, and Biological Frontage. United States Patent Application 20110318819.
Paris
Keywords: Organohalide respiration, Microbial reductive dechlorination, PCE, Acidification, pH buffer, Silicate minerals, bioremediation
MULTIFLOW peristaltic pump was used to evaluate the extraction of lead(II), chromium(III) and copper(II) on a novel adsorbent
Behruz Barfi & Maryam Rajabi & Mahboubeh Morshedi Zadeh & Mehrorang Ghaedi & Masoud Salavati-Niasari &, Extraction of ultra-traces of lead, chromium and copper using ruthenium nanoparticles loaded on activated carbon and modified with N,N-bis-(?-methylsalicylidene)-2, 2-dimethylpropane-1,3-diamine, Microchimica Acta, online: 1436-5073.
Semnan University, Iran; Yasouj University, Iran; Kashan University, Iran and Ilam University, Iran.
Keywords: Ruthenium nanoparticles loaded on activated carbon; N,N-bis-(?-methylsalicylidene)-2,2-dimethylpropane-1,3-diamine; Metals; Plasma; Food; Water
{slider Nanotechnology}
Sample additions into the silica microbeads packed optically transparent silica capillary were performed using an RS485 LAMBDA Peristaltic Pump at a flow rate of 0.5 ml/ hour
Silvia Scarmagnani, Zarah Walsh, Fernando Benito Lopez, Conor Slater, Mirek Macka, Brett Paull, and Dermot Diamond, Photoswitchable Stationary Phase Based on Packed Spiropyran Functionalized Silica Microbeads, e-J. Surf. Sci. Nanotech. Vol. 7 (2009) 649-652
National Center for Sensor Research, School of Chemical Sciences, Dublin City University, Ireland
Keywords: Photoswitchable microspheres; Monolithic frit; Spiropyrans; Stationary phase; Metal ions
{slider Analytical Chemistry}
Injection of different samples using the Lambda MULTIFLOW peristaltic pumps
Stjernlöf, A. 2007. Portable capillary electrophoresis system with LED-absorbance photometric and LED-induced fluorescence detection.
Karlstad University, Sweden; Dublin City University, Ireland
Keywords: Capillary electrophoresis (CE), LED?absorbance photometric detector (LED?AP), LED?induced fluorescence (LED?IF) detector, limit of detection (LOD)
{/sliders}